Plant biotechnology, or “In vitro culture of higher plants”, is defined as the culture and manipulation of plants, embryos, organs, explants, tissues, cells and protoplasts on nutrient media under sterile conditions. Plant biotechnology has shown a spectacular development since 1975, resulting in the production and regeneration of viable individuals of many plant species. In addition, since 1980 there has been an explosion in the development of genetic manipulation techniques. In vitro culture of higher plants has become an important tool, particularly in ornamental horticulture and plant breeding. The applications can be summarized as follows: embryo culture, germination of orchid seeds, cloning of orchids, production of disease free plants, micropropagation of many plant species, somaclonal variation, in vitro fertilization, induction of haploids, genetic manipulation, transformation, and a number of miscellaneous applications (e.g. mutation induction, separation of chimaeras, isolation of mutations, chromosome doubling, storage of plants at low temperatures biosynthesis of chemicals, etc.)