Irrigating crops with saline water induces adverse effects on productivity and causes deterioration of agricultural soils. NaCl is the most important toxic salt that induces ionic and osmotic stress in plants. Consequently, plants require a greater effort to absorb water, which affects their growth. A study was conducted in a greenhouse to determine the symbiotic effects of arbuscular mycorrhizal fungi (AMF) Burize ST® and Micorriza INIFAP® (Rhizophagus intraradices) on sorghum hybrids ‘Norteño’ and ‘Gstar 7609,’ subjected to irrigation with three levels of saline water (desalinated, medium and high, EC = 0.03, 2.30 and 4.54 dS·m-1, respectively). Variables measured were chlorophyll (SPAD), plant height, stem diameter, shoot and root biomass, and mycorrhizal colonization. Except for colonization, which was not influenced by the water’s salinity level, the values of the other variables decreased as the salt concentration in the water increased. The results indicated that, in general, higher growth and biomass yield were obtained in sorghum plants by the symbiotic association between the AMF Micorriza INIFAP and the sorghum ‘Norteño’ at the three salinity levels in the irrigation water.