Optimization of a mature cotyledons-based in vitro culture system forembryogenic-callus induction in carob (Ceratonia siliqua L.)

Lozzi, Assia; Abdelwahd, Rabha; Alami-Halimi, Driss; Mentag, Rachid; Abousalim, Abdelhadi

doi:10.5154/r.rchscfa.2018.06.053

Results and discussion: All the tested genotypes showed high callus induction levels (75 to 100 %). The Gamborg medium (B5) supplemented with 2.5 µM 2,4-D produced the highest dry weight (32.5 g) of creamy white calli. The highest amount of friable creamy-white calliwas obtained in the medium supplemented with 90 mM of sucrose. Histological analysis showed the presence of meristematic centers that became embryogenic masses and globularproembryos.
Conclusion: Mature cotyledons of C. siliqua have potential for induction and proliferation ofembryonic callus. This study aims to contribute to developing an appropriate protocol formass propagation of carob.">

COORDINACIÓN DE REVISTAS INSTITUCIONALES | UACh

e-ISSN: 2007-4018 / ISSN print: 2007-3828

Revista Chapingo Serie Ciencias Forestales y del Ambiente

Vol. XXV, issue 1 2019

Scientific article
doi: 10.5154/r.rchscfa.2018.06.053
Optimization of a mature cotyledons-based in vitro culture system forembryogenic-callus induction in carob (Ceratonia siliqua L.)

Assia Lozzi; Rabha Abdelwahd; Driss Alami-Halimi; Rachid Mentag; Abdelhadi Abousalim

Keywords: Fabaceae; genotype; mineral nutrients; sucrose; histological study