Based on 24 permanent sampling plots, randomly established in the Cupressus lusitanica Mill seedling production area, at the forest nursery in Technological Institute of Costa Rica, at Cartago, the present study was conducted during 28 weeks to determine a quality control methodology for this tree species in nurseries. A random sampling method is proposed based on random plots of two rows (28 seedlings), in which 11 quantitative and qualitative variables are recorded for each seedling sampled. The results showed 1.87 % bifurcated seedlings, 0.51 % with loss of meristem, 2.21 % with severely twisted stem, 15 % in suppressed sociological position, 0.68 % with mechanical injuries, 0.51 % with severe phytopathological problems, 13.5 % with low quality roots, and finally, 3.92 % of the seedlings were grade 3, or in the rejection category. Root length increased an average of 1.39 cm/week, stem diameter 1.14 mm/week, and total height 1.3 cm/week. Mortality rate was stabilized between the 12th and 15th week after transplanting and reached an accumulative percentage of nearly 25 %. Management and genetic (hereditary) are discussed as possible causes of the quality of cypress seedlings in nurseries. Finally, values of acceptability and rejection are proposed for cypress seedling production, based on the most important variables.