The in vitro establishment and sprouting of axillary buds and shoot apex of Ginkgo biloba L. was evaluated using as a basic medium the inorganic salts of Murashige and Skoog (1962) MS, supplemented with thiamine-HCl (0.5 mg·liter-1), myo-inositol 100 mg·liter-1, 3 % sucrose, Aguilar (1993) vitamins, 2 g·liter-1 of activated charcoal, 0.7 % agar-agar, Benomyl (400 mg·liter-1), and with a pH of 6.0±0.1. In sprouting and proliferation, the medium described above was used without the addition of activated charcoal and Benomyl. Different concentrations of BA (0, 5, 10, 15 y 20 mg·liter-1) were evaluated. Incubation was done under a light intensity of 50.48-59.83 mmol·m-2·s-1 and a photoperiod of 16 h of light and 8 h of dark with a temperature of 26/24 ºC (day/night). The results demonstrated that the season of the year in which the explants were collected influences establishment, while the age of the explant and its position on the branch influence sprouting. The best responses were obtained in one-year-old explants taken from apical regions. Sprouting in the control was 25 %, contrasting with sprouting and proliferation in the 15 mg·liter-1 treatment which was 60 % and in the 20 mg·liter-1 treatment it was 80 %.

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