In Mexico, tomato ( Solanum lycopersicum L.) cultivation is of great economic and social importance; however, the generation of innovative national varieties is limited. This research aimed to determine the agronomic potential and fruit quality of 29 F5 tomato lines with indeterminate growth habit (IGH) and round fruit, for their possible commercial use and as a source of germplasm for breeding. The 29 lines and the commercial variety Caimán (used as a control) were evaluated in a hydroponic greenhouse system during two growing cycles (2020 and 2021), under a randomized complete block experimental design with three replications. Phenological data, physical and fruit quality traits, and total number and weight of fruits per plant were recorded. An analysis of variance, a comparison of means (Tukey, P ≤ 0.05) and a Pearson correlation analysis were performed. Significant differences were found among lines in days to flowering and ripening, length, diameter, weight, firmness, soluble solids content, and total number and weight of fruits. Outstanding lines were identified in earliness, yield and fruit quality, making them suitable for fresh consumption. Twenty-eight genotypes showed no significant differences in yield with respect to the control, while line 21136 outperformed it with a 34.9 % higher yield (P ≤ 0.05), suggesting its potential to be used as a commercial variety or as a source of germplasm in breeding programs.