Keywords
germplasm characterization
molecular ISSR profiles
How to Cite
Abstract
Husk tomato is an important crop that has been subjected to a wide range of domestication process all across Mexico; therefore, there is an extensive genetic variability available. Consequently, it is convenient to characterize molecularly commonly cultivated varieties that are used as reference to classify new accessions or to register new improved varieties. Accordingly, 12 husk tomato varieties belonging to eight different races were fingerprinted using ISSR (Inter Simple Sequence Repeat) markers. Twenty-two ISSR primers produced a total of 208 bands, and 172 of them were polymorphic (representing 83 % of polymorphism). DNA fingerprinting table was compiled. Six primers were absolutely required for analyzing tested cultivar cultivars. UBC-835, UBC-873, and UBC-823 primers showed high discernment power, and therefore they will be very useful in further on genetic studies of this crop. In addition, Jaccard's distances between pairs of races were used to create a dendrogram. The results obtained showed that ISSR molecular markers can be used for reliable identification of husk tomato varieties.
References
Choi J. K., Murillo, G., Bao-Ning, S., Pezzuto, J. M., Kinghorn, A. D., & Meht, R. G. (2006). Ixocarpalactone A isolated from the Mexican tomatillo shows potent antiproliferative and apoptotic activity in colon cancer cells. FEBS J. 273:5714-5723. doi https://doi.org/10.1111/j.1742-4658.2006.05560.x
De la Cruz, M., Ramírez, F., & Hernández, H. (1997). DNA isolation and amplification from cacti. Plant Molecular Biology Reporter 15: 319-325. doi https://doi.org/10.1023/A:1007428818078
Escobar-Guzmán, R. E., Hernández-Godínez, F., Martínez de la V., O., & Ochoa-Alejo, N. (2009). In vitro embryo formation and plant regeneration from anther culture of different cultivars of Mexican husk tomato (Physalis ixocarpa Brot.). Plant Cell Tiss. Org. Cult. 96:181-189. doi https://doi.org/10.1007/s11240-008-9474-x
FAO (2014). FAO Statistical Yearbook 2014. World food and agriculture. Food and Agriculture Organization of the United Nations. Rome. http://www.fao.org/3/a-i3592e.pdf
Gill L., H. R., Rosales C., R., Hernández D., S., & Mayek P., N. (2014). Morphological and molecular characterization of common bean landraces cultivated in the semi-arid Mexican high plateau. Bol. Soc. Argent, Bot. 49(4):525-540. doi https://doi.org/10.2135/cropsci2004.0501
Johnson, D.E. 2000. Métodos Multivariados Aplicados al Análisis de Datos. International Thomson Editors. 566 pps.
Ponce-Valerio, J. J., Peña-Lomelí, A., Sánchez-del-Castillo, F., Rodríguez-Pérez, J. E., Mora-Aguilar, R., Castro-Brindis, R. & Magaña-Lira, N. (2011). Evaluation
of pruning in two husk tomato (Physalis ixocarpa Brot. ex Horm.) varieties cultivated in field. Revista Chapingo Serie Horticultura 17(3):151-160. doi https://doi.org/10.5154/r.rchsh.2011.17.026
Santiaguillo H., J. F., Vargas P., O., Grimaldo J., O., Magaña L., N., Caro V., J. F., Peña L., A. & Sánchez M., S. (2012). Diagnóstico del Tomate de Cáscara. Universidad Autónoma Chapingo. 46 p. https://www.researchgate.net/publication/283492117_Diagnostico_del_Tomate_de_Cascara
Sharma, N., Bano, A., Dhaliwal, H. S., & Sharma, V. (2015). Perspectives and possibilities of Indian species of genus Physalis (L.). A comprehensive review. Eur. J. Pharm. Med. Res. 2(2):326-353. https://storage.googleapis.com/journal-uploads/ejpmr/article_issue/1425120993.pdf
Tilahun, S., Paramaguru, P., & Hanna, B. J. R. (2013). Genetic diversity in certain genotypes of chili and paprika as revealed by RAPD and SSR analysis. Asian J. Agric. Sci. 5(2):25-31. doi https://doi.org/10.19026/ajas.5.2580
Unión Internacional para la Protección de las Obtenciones Vegetales (UPOV). 2007. Tomate de cáscara. Directrices para la ejecución del examen de la distinción, la homogeneidad y la estabilidad. n:orgupovsharedtghusk tomatotg_236_1_s.doc
Vargas-Ponce, O., Pérez-Alvares, L. F., Zamora-Tavares, P., & Rodríguez, A. (2011). Assessing genetic diversity in Mexican husk tomato species. Plant Mol. Biol. Rep. 29:733-738. doi https://doi.org/10.1007/s11105-010-0258-1
Unión Internacional para la Protección de las Obtenciones Vegetales (UPOV). 2007. Tomate de cáscara. Directrices para la ejecución del examen de la distinción, la homogeneidad y la estabilidad. n:orgupovsharedtghusk tomatotg_236_1_s.doc
USDA (2015). Tomatillo. National nutrient database for standard reference release 27. http://ndb.nal.usda.gov/ndb/foods/show/3598.
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