Keywords
Pleurotus ostreatus
exhausted substrate (Thesaurus)
anthelmintic resistance
Categories
How to Cite
Abstract
The high prevalence of parasitic infections caused by gastrointestinal nematodes (GIN) negatively impacts production parameters, the availability of animal protein, and the global sheep farming economy. The growing resistance of parasites to commercial anthelmintics tends to worsen this problem. Consequently, cost-effective and sustainable control strategies are needed that can target the various developmental stages of these parasites. The objective of this study was to evaluate the ovicidal and larvicidal effects of mushrooms and spent substrate from two strains of Pleurotus ostreatus against GIN in sheep. To obtain GIN eggs and larvae, fecal samples were collected from 20 animals in a previously diagnosed flock from the municipality of Ayapango, Estado de México. The in vitro effect of four concentrations of mushrooms and spent substrate (1.2 %, 2.25 %, 4.50 %, and 9 %) was evaluated in triplicate. Distilled water was used as a negative control and 1 % ivermectin as a positive control. Exposure times were 72 hours for eggs and 24 and 48 hours for larvae. Statistical analysis was performed using ANOVA. The highest average mortality rate in egg hatch inhibition was observed at the 9 % concentration, with 78.3 % for mushrooms and 69.3 % for the spent substrate. For larvae, the same 9 % concentration after 48 hours resulted in average mortalities of 65.6 % and 65.2 %, respectively. The use of P. ostreatus and its spent substrates represent a viable option for reducing reliance on commercial anthelmintics, minimizing environmental damage, and promoting sustainable sheep farming.
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