Abstract
The control of Rhipicephalus sanguineus is vital to prevent discomfort in domestic dogs as well as to reduce the risk of transmission of several zoonotic pathogens. Increased resistance and inefficiency of the most extended synthetic acaricides demonstrate the need to study into novel, effective and environmentally safe treatment options for brown
dog tick control. This study aims to evaluate the acaricide activity of eugenol in vitro on
R. sanguineus sensu stricto obtained in Argentina. Nine R. sanguineus s.s. populations from three provinces (San Luis, Buenos Aires, and Río Negro) were evaluated. The larval packet test was used to assess the toxicological response of R. sanguineus s.s. to eugenol. Dose–response mortality regressions, lethal concentrations at 50% (LC50), and slope were calculated by probit analysis. The concentrations that killed 50% of the tick populations studied were those with concentrations between 3.20 and 7.71 mg·mL-1. In conclusion, eugenol has good acaricidal activity against the larvae of R. sanguineus s.s., and substantial inter-population variation in response to eugenol was found in the nine tick populations studied.
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