Keywords
Zadiprion
Neodiprion
Monocteus
bioinsecticides
How to Cite
##article.highlights##
- The first search for baculoviruses in sawflies is presented.
- Ten populations of eight sawfly species of Mexico yielded eleven samples baculoviral inocula.
- A hybridization test confirmed the presence of baculoviral DNA in our samples.
- Two of the five tested baculoviral inocula exhibited high potential as bioinsecticides.
- Rictus mortem of sawflies infected with the baculovirus differs from that of lepidopterans.
Abstract
Introduction: Sawflies (Hymenoptera: Diprionidae) affect conifers and other forest species in the northern hemisphere, reducing forest productivity and causing stand death, thereby justifying control actions.
Objective: The aim was to collect baculoviral strains from Mexican sawfly populations and explore their potential
for developing biopesticides.
Materials and methods: A search for baculovirus-infected field populations was carried out. Twenty-three samples of live or dead larvae were collected from 12 localities. Microscopic diagnosis at 400X or 1000X under phase contrast, a DNA hybridization test, and a pathogenicity test were performed.
Results and discussion: Samples from eleven localities showed baculoviral polyhedra. Three subsamples of
Zadiprion ojedae DNA from Guachochi, Chihuahua, hybridized with a synthetic probe of the Neodiprion sertifer Polh
gene sequence, confirming they harbored baculovirus infection.
Five tested inocula produced disease and mortality in all treated larvae; two of them induced baculoviral polyhedra proliferation in ≥ 89 % of the resulting cadavers. The rictus mortem of sawfly larvae killed by baculovirus differs from that observed in Lepidoptera: most baculovirus-bearing cadavers remain firm or hard, flattened or not, obscured or not, and do not hang from the hind legs. For most of the cadavers found with liquefied tissues, the agent that most probably caused death was not the baculovirus but the accompanying microbiota (bacilli, cocci, or fungi).
Conclusions: Baculovirus strains are widely present in Mexican sawfly populations and several of them were pathogenic and promising for developing bioinsecticides.
References
Arthurs, S., & Dara, S. K. (2019). Microbial biopesticides for invertebrate pests and their markets in the United States. Journal of Invertebrate Pathology, 165, 13—21. doi: https://doi.org/10.1016/j.jip.2018.01.008
Balla, A., Silini, A., Cherif-Silini, H., Chenari Bouket, A., Moser, W. K., Nowakowska, J. A., ...Belbahri, L. (2021). The threat of pests and pathogens and the potential for biological control in forest ecosystems. Forests, 12(11), 1579. doi: https://doi.org/10.3390/f12111579
De Lira Ramos, K. V., González Gaona, E., & Sánchez Martínez, G. (2021). Características generales de las moscas sierra de las coníferas. In E. González G., & K. V. De Lira R. (Eds.), Moscas sierra: Taxonomía, fenología, distribución y manejo (pp. 35—82). Aguascalientes, México: INIFAP-CIRNOC-Campo Experimental Pabellón.
De Lira-Ramos, K. V., González-Gaona, E., Rodríguez-Cruz, Y. E., Piza-Núñez, E. G., & Gómez-Núñez, J. C. (2022). Nueva especie de Monoctenus (Hymenoptera: Diprionidae) ataca Juniperus flaccida Schltdl. (Cupressaceae) en Guerrero, México. Revista Mexicana de Ciencias Forestales, 13(69), 73-94. https://doi.org/10.29298/rmcf.v13i69.1093
Dixon, W. (2019). Pine sawflies, Neodiprion spp. (Insecta: Hymenoptera: Diprionidae). Retrieved from https://edis.ifas.ufl.edu/pdf%5CIN%5CIN59200.pdf
Federici, B. A. (1997). Baculovirus pathogenesis. In L. K. Miller (Ed.), The Baculoviruses. The Viruses (pp. 33—59). Springer, Boston, MA: Springer. doi: https://doi.org/10.1007/978-1-4899-1834-5_3
González-Gaona, E., Bonilla, T. F., Quiñones, B. S., Sánchez, M. G., Tafoya, R. F., España, L. M. P., ...Robles, U. S. (2014). Guía para la identificación de moscas sierra de la familia Diprionidae presentes en el centro norte de México. Mexico: INIFAP. Retrieved from https://docplayer.es/46918897-Guia-guiapara-la-identificacion-la-identificacion-moscas-sierra-lafamilia-diprionidae-de-presentes-moscas-en-el-centronorte-sierra.html
González-Gaona, E., Borja-Nava, HE, Lira-Ramos, D., Karla, V., Rodríguez-Cruz, YE, & Arriola-Padilla, VJ (2022). Nueva especie de mosca sierra del género Zadiprion Rohwer (Hymenoptera: Diprionidae) atacando a Pinus cembroides(Zucc.) en Tamaulipas, México. Revista Chapingo Serie Ciencias Forestales, 28 (3) 399-409.
Green, M. R., & Sambrook, J. (2012). Molecular cloning. A laboratory manual (4th ed). USA: Cold Spring Harbor Laboratory Press.
Harrison, R. L., Herniou, E. A., Jehle, J. A., Theilmann, D. A., Burand, J. P., Becnel, J. J., Krell, …Bauchan, G. R. (2018). ICTV virus taxonomy profile: Baculoviridae. Journal of General Virology, 99(9), 1185—1186. Retrieved from https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.001107?crawler=true
Hughes, D. S., Possee, R. D., & King, L. A. (1997). Evidence for the presence of a low-level, persistent baculovirus infection of Mamestra brassicae insects. Journal of General Virology, 78(7), 1801—1805. doi: https://doi.org/10.1099/0022-1317-78-7-1801
Il’inykh, A. V., & Ul’yanova, E. G. (2005). Latency of baculoviruses. Biology Bulletin, 32(5), 496—502. doi: https://doi.org/10.1007/s10525-005-0131-1
Lauzon, H. A., Garcia-Maruniak, A., Paolo, M. D. A., Clemente, J. C., Herniou, E. A., Lucarotti, C. J., ... Maruniak, J. E. (2006). Genomic comparison of Neodiprion sertifer and Neodiprion lecontei nucleopolyhedroviruses and identification of potential hymenopteran baculovirus-specific open reading frames. Journal of General Virology, 87(6), 1477—1489. doi: https://doi.org/10.1099/vir.0.81727-0
Lucarotti, C. J., Morin, B., Graham, R. I., & Lapointe, R. (2007). Production, application, and field performance of Abietiv™, the balsam fir sawfly nucleopolyhedrovirus. Virologica sinica, 22(2), 163. doi: https://doi.org/10.1007/s12250-007-0018-z
Meier-Kolthoff, J. P., Hahnke, R. L., Petersen, J., Scheuner, C., Michael, V., Fiebig, A., ... Klenk, H. P. (2014). Complete genome sequence of DSM 30083 T, the type strain (U5/41 T) of Escherichia coli, and a proposal for delineating subspecies in microbial taxonomy. Standards in Genomic Sciences, 9(1), 1—19. doi: https://doi.org/10.1186/1944-3277-9-2
Moreau, G., & Lucarotti, C. J. (2007). A brief review of the past use of baculoviruses for the management of eruptive forest defoliators and recent developments on a sawfly virus in Canada. The Forestry Chronicle, 83(1), 105—112. doi: https://doi.org/10.5558/tfc83105-1
Moreau, G., Lucarotti, C. J., Kettela, E. G., Thurston, G. S., Holmes, S., Weaver, C., …Morin, B. (2005). Aerial application of nucleopolyhedrovirus induce decline in increasing and peaking populations of Neodiprion abietis. Biological Control, 33(1), 65—73. doi: https://doi.org/10.1016/j.biocontrol.2005.01.008
Nolasco-Gumeta, A. (2014). Defoliadores de coníferas de los géneros Zadiprion spp. y Neodiprion spp. existentes en México. Tesis profesional. Departamento Forestal, División de Agronomía, UAAAN. Saltillo, Coahuila, México. Retrieved from http://repositorio.uaaan.mx:8080/xmlui/handle/123456789/3887
Olivo, M. J. A. (2011). Brotes epidémicos de diprionidos en la sierra Tarahumara de Chihuahua. M. A. Equihua, & V. Estrada E., J. A. Acuna S., & M. P. Cháires G. (Eds.), XV Simposio Nacional de Parasitología Forestal. Oaxaca, México: CONAFOR. Retrieved from https://www.uaeh.edu.mx/investigacion/icap/LI_IntGenAmb/Juana_Fons/8.pdf
Qinghua Wang, Enjie Li, Na Li, Yuzhu Wang, Zhilin Zhang, & Yongan Zhang. (2018). Infection of a nucleopolyhedrovirus to Neodiprionzhejiangenis Zhou & Xiao (Hymenoptera: Diprionidae). Biocontrol Science and Technology, 28(8), 761—771. doi: https://doi.org/10.1080/09583157.2018.1493089
Smith, D. R. (1988). A synopsis of the sawflies (Hymenoptera: Symphyta) of America south of the United States: introduction, Xyelidae, Pamphiliidae, Cimbicidae, Diprionidae, Xiphydriidae, Siricidae, Orussidae, Cephidae. Systematic Entomology, 13(2), 205—261. doi: https://doi.org/10.1111/j.1365-3113.1988.tb00242.x
Smith, D. R. (1993). Systematics, life history and distribution of sawflies. In M. R. Wagner, & K. F. Raffa (Eds.), Sawfly life history adaptations to wood plants (pp. 3— 32). California, USA: Academic Press. Retrieved from https://www.researchgate.net/publication/260798522_Systematics_life_history_and_distribut9ion_of_sawflies#fullTextFileContent
Smith, R. D., Sánchez-Martínez, G., & Ordaz-Silva, S. (2010). A new Monoctenus (Hymenoptera: Diprionidae) damaging Juniperus flaccida (Cupressaceae) in San Luis Potosí, México. Proceedings of the Entomological Society of Washington, 112(3), 444–450. doi: https://doi.org/10.4289/0013-8797.112.3.444
Tapia-Uriza, T. R., Cossío-Bayúgar, R., González-Gaona, E., LiraRamos, K. V. D., Rodríguez-Cruz, Y. E., & Miranda-Miranda, E. (2022). Establecimiento de novo de un cultivo in vitro de una línea celular derivada del intestino de moscas sierra (Hymenoptera: Diprionidae). Revista Mexicana de Ciencias Forestales, 13(69), 95—111. doi: https://doi.org/10.29298/rmcf.v13i69.1088
Thompson, C. G., Scott, D. W., & Wickman, B. E. (1981). Longterm persistence of the nuclear polyhedrosis virus of the Douglas-fir tussock moth, Orgyia pseudotsugata(Lepidoptera: Lymantriidae), in forest soil. Environmental Entomology, 10(2), 254—255. doi: https://doi.org/10.1093/ee/10.2.254
van Frankenhuyzen, K., Lucarotti, C., & Lavallée, R. (2016). Canadian contributions to forest insect pathology and to the use of pathogens in forest pest management. The Canadian Entomologist, 148(S1), S210—S238. doi: https://doi.org/10.4039/tce.2015.20
Williams, T., Virto, C., Murillo, R., & Caballero, P. (2017). Covert infection of insects by baculoviruses. Frontiers in Microbiology, 8, 1337. doi: https://doi.org/10.3389/fmicb.2017.01337
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