Keywords
cankers
endophyte
Eucalyptus sp.
die-back
How to Cite
Abstract
In 2013, in the east of Michoacán, die-back in commercial eucalyptus plantations appeared in trees with frost and drought damage. Neofusicoccum eucalyptorum was isolated on Eucalyptus nitens, and its teleomorph Botryosphaeria eucalyptorum was found on cankers; N. parvum isolated on E. nitens and E. globulus. Both species of Neofusicoccum were identified by morphology and were molecularly characterized by PCR-ITS. The sequences of N. eucalyptorum (access number: KC479184 and KC4799188) and N. parvum (KC479185, KC479186 and KC479187) were deposited in the Gene Bank of the NCBI. In the field, diseased eucalyptus showed die-back, fusiform cankers on stem, which induce bulges, resin flow and the appearance of epicormic shoots. The fungi inoculated in vitro in shoots of E. nitens and E. globulus provoked necrotic lesions and abundant immature pycnidia at 10 days after inoculation (dai). E. nitens trees of three years of age showed a fusiform canker with sunken appearance and dark brown coloration, measuring 13 to 21.9 cm at 48 dai. This study is the first report of N. eucalyptorum and N. parvum in Mexico causing disease in eucalyptus plantations.
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