Keywords
functional foods
active secondary metabolites
How to Cite
Abstract
Five compounds in a commercial strain of the mushroom Pleurotus sp. were obtained from ethyl acetate and methanol extracts. The compounds were isolated using techniques such as column and thin layer chromatography. The following sterols were identified by 1H NMR spectroscopy: 1) ergosta-5,7,22-trien-3β-ol (ergosterol), 2) 5α, 8α-epidioxy-22E ergosta-6, 22-dien-3β-ol (ergosterol peroxide), 3) 3β,5α,6β,9α-tetrahidroxiergosta-7,22 dieno, 4) 3β, 5α, 6β, 9α-tetrahydroxyergosta-7, 22-dien and 5) 3β, 5α, 9α trihidroxiergosta-7,22-dien-6-one.
References
Alarc.n, J., .guila, S., Arancibia-.vila, P., Fuentes, O., Zamorano- Ponce, E., & Hern.ndez, M. (2003). Production and purification of statin from Pleurotus ostreatus (basidiomycetes) strains. Zeitschrift für Naturforschung C, 58, 62–64.
Bok, J. W., Lermer, L., Chilton, J., Klingeman, H. G., & Towers, G. H. N. (1999). Antitumor sterols from the mycelia of Cordyceps sinensis. Phytochemistry, 51, 891–898. doi: https://doi.org/10.1016/S0031-9422(99)00128-4
Chegwin-Angarita, C., Nieto-Ram.rez, I., Atehort.a, L., & Sep.lveda, A. L. (2012). Las estatinas: Actividad biol.gica y producci.n biotecnol.gica. Revista Colombiana de Biotecnología, 14(2), 157–178. http://www.revistas.unal.edu.co/index.php/biotecnologia/article/view/37418/39788
Cohen, R., Persky, L., & Hadar, Y. (2002). Biotechnological applications and potential of wood-degrading mushrooms of the genus Pleurotus. Applied Microbiology Biotechnology, 58, 582–594. doi: https://doi.org/10.1007/s00253-002-0930-y
Eriksson, K. E. L., Blanchette, R. A., & Ander, P. (1990). Microbial and enzymatic degradation of wood and wood components. Berlin, Germany: Springer- Verlag
Ghorai, S., Prosad, B. S., Verma, D., Chowdhury, S., Mukherjee, S., & Khowala, S. (2009). Fungal biotechnology in food and feed processing. Food Research International, 42, 577–587. doi: https://doi.org/10.1016/j.foodres.2009.02.019
Kawagishi, H., Katsumi, R., Sazawa, T., Mizuno, T., Hagiwara, T., & Nakamura, T., (1988). Cytotoxic steroids from the mushroom Agaricus blazei. Phytochemistry, 27(9), 2777– 2779. doi: https://doi.org/10.1016/0031-9422(88)80662-9
Kobori, M., Yoshida, M., Ohnishi-Kameyama, M., & Shinmoto, H. (2007). Ergosterol peroxide from an edible mushroom suppresses inflammatory responses in RAW264.7 macrophages and growth of HT29 colon adrenocarcinoma cells. British Journal of Pharmacology, 150, 209–219. doi: https://doi.org/10.1038/sj.bjp.0706972
Mizushina, Y., Takahashi, N., Hanashima, L., Koshino, H., Esumi, Y., Uzawa, J., … Sakaguchi, K. (1999). Lucidenic acid O and lactone, new terpene inhibitors of eukaryotic DNA polymerases from a basidiomycete Ganoderma lucidum. Bioorganic and Medicinal Chemistry, 7(9), 2047–2052. http://ac.els-cdn.com/S0968089699001212/1-s2.0-S0968089699001212-main.pdf
Mora, V. M., & Mart.nez-Carrera, D. (2007). Investigaciones básicas, aplicadas y socioecon.micas sobre el cultivo de setas (Pleurotus) en M.xico. En E. S.nchez, D. Mart.nez- Carrera, G. Mata, & H. Leal (Eds.), El cultivo de setas Pleurotus spp. en México (pp. 69–110). http://hongoscomestibles-latinoamerica.com/P/P/8.pdf
Ramos-Ligonio, A., L.pez-Monteon, A., & Trigos., .. (2012). Trypanocidal activity of ergosterol peroxide from Pleurotus ostreatus. Phytotherapy Research, 26(6), 938–943. doi: https://doi.org/10.1002/ptr.3653
Subbiah, M. T. R., & Abplanalp, W. (2003). Ergosterol (major sterol of baker’s and brewer’s yeast extracts) inhibits the growth of human breast cancer cells in vitro and the potential role of its oxidation products. International Journal for Vitamin and Nutrition Research, 73, 19–23. doi: https://doi.org/10.1024/0300-9831.73.1.19
Takaku, T., Kimura, Y., & Okuda, H. (2001). Isolation of an antitumor compound from Agaricus blazei Murill and its mechanism of action. Journal of Nutrition. 131, 1409–1413. http://jn.nutrition.org/content/131/5/1409.full.pdf+html
Trigos, .., Amezcua, C., Reyna, S., & Carri.n, G. (1997). Cerevisterol from cultures of Verticilium lecani. Micologia Neotropical Aplicada, 10, 56–62.
Trigos, .., & Ortega-Regules, A. (2002). Selective destruction of microscopic fungi through photo-oxidation of ergosterol. Mycologia, 94(4), 563–568. http://www.mycologia.org/content/94/4/563.full.pdf+html
Trigos, .., & Su.rez, J. (2010). Los hongos como alimentos funcionales y complementos alimenticios. En D. Mart.nez- Carrera, N. Curvetto, M. Sobal, P. Morales, & V. M. Mora (Eds.), Hacia un desarrollo sostenible del sistema d e producción-consumo de los hongos comestibles y medicinales en Latinoamérica: Avances y perspectivas en el siglo XXI (pp. 59–76). Puebla, M.xico: Red Latinoamericana de Hongos Comestibles y Medicinales-COLPOS-UNS-CONACYTAMC- UAEM-UPAEP-IMINAP.
Trigos, .., Zayas, T., Ortu.o, L., Sobal, M., & Morales, P. (1994). Contenido de ergosterol en algunas especies cultivadas de Pleurotus. Micologia Neotropical Aplicada, 7, 43–46.
Tuor, U., Winterhalter, K., & Fiechter, A. (1995). Enzymes of whiterot fungi involved in lignin degradation and ecological determinants for wood decay. Journal of Biotechnology, 41, 1–17. http://ac.els-cdn.com/016816569500042O/1-s2.0-016816569500042O-main.pdf
Wasser, S. P. (2011). Current findings, future trends, and unsolved problems in studies of medicinal mushrooms. Applied microbiology and biotechnology, 89,1323–1332. doi: https://doi.org/10.1007/s00253-010-3067-4
Wiseman, H. (1993). Vitamin D is a membrane antioxidant: Ability to inhibit iron-dependent lipid peroxidation in liposomes compared to cholesterol, ergosterol and tamoxifen and relevance to anticancer action. FEBS Letters 326, 285–288. http://ac.els-cdn.com/001457939381809E/1-s2.0-001457939381809E-main.pdf
Yazawa, Y., Yokota, M., & Sugiyama, K. (2000). Antitumor promoting effect of an active component of Polyporus, ergosterol and related compounds on rat urinary bladder carcinogenesis in a short-term test with concanavalin A. Biology & Pharmacology Bulletin, 23, 1298–1302. doi: https://doi.org/10.1248/bpb.23.1298
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Copyright (c) 2014 Revista Chapingo Serie Ciencias Forestales y del Ambiente