Keywords
callus
necrosis
morphogenesis
How to Cite
Abstract
Necrosis and morphogenic response of cotyledon, epicotyl and hypocotyl explants of Lupinus montanus (HBK) seedlings germinated in vitro were evaluated to establish the basic conditions of micropropagation. Necrosis was evaluated in MS medium with 0.40 mg·liter-1 thiamine, 100 mg·liter-1 myo-inositol, 3 % sucrose and 7 g·liter-1 agar-agar and different doses of activated carbon (AC) and citric acid. Treatments with AC (50 mg·liter-1 and 100 mg·liter-1) were the best in the control of necrosis (P = 0.001); the cotyledon had a higher level of necrosis (< 60 %) than the epicotyl and hypocotyl (10 to 30 %). Morphogenic responses were analyzed on MS medium with 0.40 mg·liter-1 thiamine, 100 mg·liter-1 myo-inositol, 3 % sucrose, 7 g·liter-1 agar-agar, 100 mg·liter-1 AC and five combinations of IAA (indole acetic acid) and BA (6-benzyladenine). The hypocotyl and epicotyl explants showed higher organogenic response (> 70 %), whereas the cotyledon response was primarily callogenic (50 %). The epicotyl cultured on MS medium with 3.0 μM IAA and 1.0 μM BA had the highest number of shoots (10) and height thereof (11.4 ±2.6 cm).
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