Keywords
chemolithoautotrophic
chemoheterotrophic
bioremediation
How to Cite
Abstract
Arsenic is one of the most toxic metalloids present in the environment and prolonged exposure to this metal causes chronic health effects. Therefore, the search for environmentally-friendly alternatives for the treatment of arsenic-contaminated water and soil is important. In this study, bacterial strains were isolated from arseniccontaining soils in the Lagunera region to analyze those with arsenite-oxidizing abiliity. Strains 04-SP1qa and 14-SP1qh with chemolithoautotrophic and chemoheterotrophic metabolism, respectively, had greater activity of the arsenite oxidase enzyme. The optimum growth conditions and enzymatic activity of these strains were investigated. Strain 04-SP1qa had specific enzymatic activity of 0.162 μmol·min-1·mg-1, Michaelis-Menten constant (Km) of 3.37 μM and maximum velocity (Vmax) of 5.2 μM·min-1·mg-1 under optimum growth conditions of pH 8.0 at 40 °C. Strain 14-SP1qh showed specific enzymatic activity of 0.16 μmol·min-1·mg-1, Km of 3.7 μM and Vmax of 14.39 μM·min-1·mg-1 at pH 7.0 and 40 °C. Results of this study demonstrated the presence of arsenite- oxidizing bacteria with enzymatic activity in the soils of the Lagunera region. Thus, the potential exists to develop new bioremediation technologies for treatment of arsenic-contaminated water and soils in the region using native bacterial strains.
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