The objective of this study was to select, among four commonly used growth media for in vitro propagation of orchids, a medium to improve in vitro germination of immature seeds and development of proto-bulbs and seedlings, for up to eight months, in three epiphytic orchid species common to the Soconusco region, Chiapas: Cattleya aurantiaca, Encyclia chacaoensis and Brassavola nodosa, orchids with potential as ornamental plants. For evaluation we identified six variables, germination, proto-bulb and seedling development stages, number of leaves, number of roots, color and size, as indicators of growth medium contribution. Germination percentage for Brassavola nodosa was similar in all growth media, but proto-bulb and seedling development was better in two media: Knudson C (macronutrimentos Knudson C, [replacing FePO4.4H2O with FeSO4.7H2O and Na2 EDTA.2H2O]; micronutrients Knudson C) and Dalla Rosa and Laneri (macronutrients Dalla Rosa and Laneri KO7, [replacing FePO4.4H2O with FeSO4.7H2O and Na2 EDTA.2H2O]; micronutrients Dalla Rosa y Laneri KO7). Cattleya aurantiaca responded better to the Dalla Rosa and Laneri medium in all stages. For Encyclia chacaoensis, in the beginning all media darkened, and germination and the first development stages were better in the Hutner (Hutner macro- and micronutrients) and Knudson C media. At 8 months, Dalla Rosa and Laneri, followed by Hutner + activated carbon, provided best results for color, size, number of leaves and number of roots in proto-bulbs and seedlings. This study is an important part of a multidisciplinary project dedicated to the conservation of orchids and in search of new economically rentable products for coffee producers affected by the low price of their product in the Soconusco region, Chiapas, Mexico.