The use of in vitro cultivation of vegetable cells and tissue to obtain somatic embryos is considered to be an efficient propagation method for a number of important agronomical and horticulture species. Among these is Solanum tuberosum L. The main objective of this study was to obtain embryogenetic cells from explants from potato leaf mesophyll, cultivars Fritolay-1867 and Atlantic. Formation of somatic embryos of Fritolay-1867 was promoted when, in the culture medium, thidiazuron was used at a concentration of 1.0 mg·liter-1 was used as a growth regulator. The embryos were cultivated in a Murashige and Skoog medium complemented with vitamins, carbohydrates, and antioxidants, and initially incubated during eight weeks of darkness and later with photoperiods of 16 h using 44 mmol·s-1·m-2 light intensity at a temperature of 26±1 °C for 60 days. At nine weeks, the first indication of embryonic germination was obtained with 5 % and development of complete plants.

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