<em>In vitro</em> PROPAGATION OF PAPA RATONA (<em>Oxalis tuberosa</em> Mol)

In order to determine the appropriate suitable culture medium for mass microprapagation of in vitro Oxalis tuberosa Mol., apical and axillary buds were cultivated in the MS basic medium incorporating benciladenine (BA) 1.0 mg×liter-1 and 0, 0.1 and 1.0 mg·liter-1 naftalenacetic acid (ANA). The incubation conditions were 16 hours of photoperiod, 20 ºC of temperature and 90 mmol (quantum) m-2×s-1 of light intensity. The ratio 1:1 of growth regulators ANA:BA, promoted a rate of multiplication of nine shoots per explant cultivated. After 31 days vigorous shoots of three centimeters of lengh were observed. The basal callus production was developed in the explants after 56 days; they were not organogenic. The shoots were transferred to a root promoting medium MS with indolbutiric acid (AIB) 0.5 mg×liter-1. Rooting occurred after 15 days. The period to obtain new plants from the explants cultivated was 155 days.

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Revista Chapingo Serie Ciencias Forestales y del Ambiente es publicada bajo una Licencia Creative Commons Atribución-NoComercial-SinDerivadas 4.0 Internacional.

Electronic ISSN: 2007-4034
Print ISSN: 1027-152X

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