The use of biofertilizers in orchid growing can be an alternative to reduce the environmental

impact of traditional inorganic fertilization. The aim of this research was to evaluate the

effect of mineral fertilization and biofertilization on some physiological parameters of

Laelia anceps Lindl. subsp. anceps seedlings in vegetative stage. Three fertilization treatments were evaluated: 1) mineral fertilization (MF), 2) biofertilization (BFERT) and 3) mineral fertilization + biofertilization (MF + BFERT). The concentration and content of chlorophylls, and the concentration of amino acids and total soluble proteins were measured. No treatment affected the concentration of chlorophylls in leaves. In general, the MF and BFERT treatments significantly increased the concentration of chlorophylls a, b and total in pseudobulbs, and their content in leaves and pseudobulbs. The concentration of amino acids in pseudobulbs was higher with MF, while the concentration of proteins in leaves was greater with MF + BFERT. Biofertilization promoted positive responses in the physiological parameters evaluated in comparison with mineral fertilization; therefore, it is considered to have potential for use in the species evaluated in this study.